Антитела. Методы. Книга 1 - Кэтти Д.
ISBN 5-03-002363-1
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Biotechnology, Pharmacia House, Midsummer Boulevard, Milton Keynes MK9
3HP, UK; Biorad Laboratories Ltd, Caxton Way, Holywell Industrial Estate,
Watford, Herts WDL 8RP, UK
18-997
274
Приложение
/
т-
Nitrocellulose membrane-Hybond С: Amersham International pic, Lincoln
Place, Green End, Aylesbury, Bucks №29 2TP, UK
К-Ray film and cassettes; Agfa Curix RP-1: DuPont Cronex; DuPont
Instruments, Peck's Lane, Newton, CT 06470, USA
Материалы, химические реактивы и реагенты Адъюванты
Arlacel A: Sigma Chemical Co. Ltd, Fancy Road, Poole, Dorset BH17 7NG, UK
Bayol F: Esso Petroleum Co., Purfleet, Essex, UK B. pertussis vaccine:
Wellcome Diagnostics, Temple Hill, Dart-ford, Kent DAI 5AH, UK FCA and
FIA: Difco Laboratories Ltd, PO Box 14B, Central Avenue, East Molesey,
Surrey KT8 OSE, UK FCA can be provided with M. tuberculosis (H37Ra) or
with M. butyricum
Liposomes; Lecithin/Sphingomyelin standard solution: Sigma
Chemical Co. Ltd, Fancy Road, Poole, Dorset BH17 7NG, UK
M. bovis BCG vaccine: Glaxo Laboratories Ltd, Greenford, Essex, UK
M. tuberculosis-. Ministry of Agriculture Veterinary Research Station
Weybridge, Surrey, UK and MRC Microbiological Supplies Division, Porton
Down, Wiltshire, UK Muramyl dipeptide: Sigma Chemical Co. Ltd, Fancy
Road, Poole, Dorset BH17 7 NG, UK
Антисыворотки, МКА и антигенные стандарты
These suppliers include those offering HRP-antiglobulin conjugates for
isotype screening.
Antiserum catalogue: Linscott Directory of Immunological and Biological
Reagents, PO Box 55, East Grinstead, Sussex RH19 3YL, UK
Anti-SRBC (rabbit haemolytic and agglutinating reagents): Wellcome
Diagnostics, Temple Hill, Dartford, Kent DAI 5AH, UK
BDS Biologicals Ltd, Vincent Drive, Edgbaston, Birmingham B15, UK
Behring Diagnostics, Hoechst UK Ltd, Hoechst House, Salisbury Road,
Hounslow TW4 6JH, UK Dako Ltd, 22 The Arcade, The Octagon, High Wycombe,
Приложение
275'
Bucks НР11 2HT, UK
Miles Laboratories Ltd, PO Box 37, Stoke Court, Stoke Po-ges, Slough SL2
4LY, UK
Nordic Immunological Labs, PO Box 544, Maidenhead, Berks SL6 2PW, UK
Serotec Ltd, 22 Bankside Approach, Kidlington, Oxford 0X5 IJE, UK
Oxoid Limited, Wade Road, Basingstoke, Hampshire RG24 OPN, UK
WHO standard serum (for serum protein standards): WHO International
Reference Centre for Immunoglobulins, Institute of Biochemistry,
University of Lausanne, Lausanne, Switzerland
Хроматографические гели
Cellulose carbonate: Sigma Chemical Co. Ltd, Fancy Road, Poole..
Dorset BH17 7NG, UK DE-52 (DEAE): Whatman Biochemicals Ltd, Springfield
Mill, Maidstone, Kent, UK Protein A-Sepharose-4B: Pharmacia/LKB
Biotechnology, Pharmacia House, Midsummer Boulevard, Milton Keynes MK9
3HP, UK
Sephadex (G25, 200 etc.), Sephacryl (S200, 300): Pharmacia/ /LKG
Biotechnology, Pharmacia House, Midsummer Boulevard, Milton Keynes MK9
3HP, UK Sepharose 4B, 6B and CL-4B: Pharmacia/LKB Biotechnology
Ферменты
HRP, papain, pepsin urease: Sigma Chemical Co. Ltd, Fancy Road, Poole,
Dorset BH17 7NG, UK
Материалы для конъюгации гаптенов с носителями
(i) Carriers
Haemocyanin (Keyhole limpet [KLH] and Limulus): Sigma Chemical Co. Ltd,
Fancy Road, Poole, Dorset BH17 7NG, UK Species serum albumins and gamma
globulins, porcine thy-roglobulin: Sigma Chemical Co. Ltd
Ficoll: Pharmacia/LKB Biotechnology, Pharmacia House, Midsummer
Boulevard, Milton Keynes MK9 3HP, UK
(ii) Haptens
DNP and TNBS: Sigma Chemical Co. Ltd
(iii) Linking agents
276
Приложение
Glutaraldehyde; water-soluble carbodiimide( N,N'-0-pheny-lenedimaleimide;
SPDP: Sigma Chemical Co. Ltd Chromic chloride (chromium chloride, CrCl3):
Sigma Chemical Co. Ltd
CNBr: Sigma Chemical Co. Ltd
Материалы для проведения ИЭФ в ПААГ в присутствии ДСН
Gels, buffers, molecular weight markers, protein stains etc: Phar-
macia/LKB Biotechnology, Pharmacia House, Midsummer Boulevard, Milton
Keynes MK9 3HP, UK Silver staining kit: Biorad Laboratories Ltd, Caxton
Way, Holy-well Industrial Estate, Watford, Herts WD1 8RP, UK Non-fat milk
powder: St Ivel 5 pints (or similar brand): Grocery Stores and
Supermarkets
Клетки, культуральные среды и их компоненты
В95-8 marmoset cell line: from Dr J. Gordon, Department of Immunology,
Medical School, University of Birmingham, Birmingham В15 2TJ, UK DMSO:
Sigma Chemical Co. Ltd, Fancy Road, Poole, Dorset BH17 7NG, UK
Dulbecco's medium: Oxoid Ltd, Wade Road, Basingstoke, Hampshire RG24 OPN,
UK Dulbecco's A+B buffer: Oxoid Ltd
Fetal calf serum (FCS): Gibco Europe Ltd, Unit 4, Cowley Mill Trading
Estate, Longbridge Way, Uxbridge UB8 27G, UK Hepes: BDH Ltd, Four Ways,
Atherstone, Warwick CV9 IJQ, UK Hepes-RPMI medium (H-RPMI): Gibco Europe
Ltd, UK Horse serum: Gibco Europe Ltd, UK Hypoxanthine: BDH Ltd, UK
2-Mercaptoethanol (2-ME): Sigma Chemical Co. Ltd, Fancy Road, Poole,
Dorset BH17 7NG, UK Methotrexate (aminopterin): Lederle Laboratories
Division, American Cyanamid Company, Pearls River, New York, USA
