Методы культивирования клеток - Вахтин Ю.Б.
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витамины
6.3Е-7 --- --- --- _ --- ¦; --- ---
1.5Е-7 --- --- --- --- --- --- _
4.5Е-8 --- --- --- --- --- --- --
8.7Е-7 - --- _ --- --- ---
и их производные
3.7Е-4 --- --- --- --- __ --- 3.7Е-3 ---
--- --- --- --- - --- --- --- ---
--- --- __ --- --- --- --- --- ---
1.8Е-5 --- --- --- --- --- --- --- ---
5.6Е-3 2.8Е-2 1.7Е-2 1.IE-2 6.1Е-3 I.0E-2 4.0Е-3 6.0Е-3 8.0Е-3
7.7Е-6 --- --- --- --- --- --- _ _
I.0E-5
--- --- - I.0E-3 1.0Е-3 1.0Е-3 5.0Е-4 5.0Е-4
--- _ --- --- --- --- --- ---
и у к л е и у О В Ы X к И С Л О т I.0E-5 I.8E-4 1.0Е-6
--- --- --- --- --- --- --- --- ---
--- --- --- --- --- --- --- --- ---
4.0Е-5 --- --- --- --- - --- --- ---
--- --- --- --- --- --- --- --- ---
3.7Е-5 --- --- --- --- --- --- --- ---
--- 1.8Е-4 --- --- З.ОЕ-5 З.ОЕ-5 --- --- ---
--- --- --- --- --- --- --- --- ---
3.8Е-5 --- --- --- --- --- --- --- ---
8.0Е-7 --- --- --- --- --- --- --- ---
--- - --- - - - - --- -
4.1Е-5 --- --- --- З.ОЕ-б З.ОЕ-б З.ОЕ-7 З.ОЕ-б З.ОЕ-7
--- --- --- --- --- --- --- --- ---
I.8E-6 --- --- --- --- --- --- --- ---
Компонент Наименование сред
МЕМ ВМЕ DME 1MDM L-I5 199 CMRL-
1066
Жиры
Холестерин --- --- --- --- --- 5.2Е-7 5.2Е-7
Холи и 8.3Е-6 I.0E-6 2.9Е-5 2.9Е-5 8.3Е-6 3.6Е-6 3.6Е-6
Ииозит 1.1Е-5 --- 3.9Е-5 4.0Е-5 1.1 Е-5 2.8Е-7 2.8Е-7
Линолеат --- --- ~ --- --- --- -
Кальций 1.8Е-3 1.0Е-3 1.8Е-3 1.5Е-3 1.3Е-3 Важ н е й ш и с
1.8Е-3 1.8Е-3
Магний 9.8Е-4 5.0Е-4 8.1Е-4 8.1 Е-4 1.8Е-3 8.1 Е-4 8.1 Е-4
Калий 5.4Е-3 5.0Е-3 5.4Е-3 4.4Е-3 5.8Е-3 5.4Е-3 5.4Е-3
Натрий 1.4Е-1 1.2Е-1 1.6Е-1 1.1Е-1 1.5Е-1 1.5Е-1 I.5E-1
Хлорид 1.3Е-1 1.1Е-1 1.2Е-1 8.7Е-2 1.5Е-1 1.3Е-1 1.3Е-1
Нитрат --- --- 7.5Е-7 7.5Е-7 --- 1.2Е-6 ---
Фосфат 9.7Е-4 1.0Е-3 9.1Е-4 1.0Е-3 7.9Е-4 1.0Е-3 1.0Е-3
Сульфат --- --- 8.IE-4 8.1Е-4 8.1 Е-4 8.6Е-4 8.1Е-4
Кобальт *** 9.6Е-9 М и к р о
Медь --- --- --- --- --- _
Железо --- 2.5Е-7 --- --- 4.1Е-7 _
Марганец --- --- --- --- --- --- ---
Молибден _ --- --- --- _ --- ---
Никель --- _ --- --- --- --- ---
Селей _ --- --- 1.0Е-7 --- --- ---
Кремний *• --- --- --- --- --- --- ---
Олово --- --- --- --- --- --- __
Ванадий --- --- --- --- --- --- ---
Цинк --- - - - - - -
Бикарбонат 2.4Е-2 2.0Е-2 4.4Е-2 3.6Е-2 1 Б у ф е р а \
2.6Е-2 2.6Е-2
Углекислый газ 5 **** g 10 5 _ ***** 8 g***«
(%> 2.5Е-2
HEPES
Феноловый 1.4Е-5 1.4Е-5 4.2Е-5 4.0Е-5 2.8Е-5 4.2Е-5 5.6Е-5
красный Про
Этанол 3.5Е-4
Твии 80 (мг/л) --- --- --- --- --- 20 5
Примечание: Состав сред приведен, в основном, по Хэму (12]; ссылки даиы в тексте; для удобства буквой Е заменено выражение «10 в степени», например, б.ОЕ-4 означает 6.0 • 10“ ; * — для удобства сравнения приведена концентрация «полуцистииа»,т. е. удвоенная концентрация цистииа; ** — отмечены DL-амииокислоты, остальные аминокислоты в L-форме; *** — кобальт входит в среды как компонент витамина В,2; **** — первоисточник ие указывает
