Клонирование ДНК. Методы - Гловер Д.
ISBN 5-03-001159-5
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2824 958 (19.1) 1 866 2824
1992 5003 (99.9) 1 992 1992
323 4530 (90.5) 323 4853
4853 4 73 ( 9.5) 4853 323
539 1 571 (31.4) 21 04 3675
21 04 1 565 (31.3) 539 2104
3675 667 (13.3) 4875 539
4337 662 (13.2) 3675 4337
4875 538 (10.8) 4337 4875
1667 3575 (71.5) 3095 1667
1 746 616 (12.3) 1 985 2601
1 763 248 ( 5.0) 2601 2849
1 985 245 ( 4.9) 2849 3094
2601 222 ( 4.4) 1 763 1 935
2849 79 ( 1.6) 1 667 1 746
3094 1 7 ( 0.3) 1 746 1 763
3095 1 ( 0.0) 3094 3095
1 95 1 145 (22.9) 2312 3457
250 1 052 (21.0) 820 1872
408 957 (19.1) 3457 441 4
524 504 (10.1 ) 441 4 4918
579 280 ( 5.6) 4918 195
600 230 ( 4.6) 2082 2312
820 220 ( 4.4) 600 820
1872 210 ( 4.2) 1872 2082
2082 1 58 ( 3.2) 250 408
231 2 1 1 6 ( 2.3) 408 524
3457 55 ( 1.1) 1 95 250
441 4 55 ( 1.1) 524 579
4918 21 ( 0.4) 579 600
1 98 4948 (98.9 ) 253 1 98
253 55 ( 1.1) 1 98 253
1 7 5003 (99.9) 1 7 1 7
832 3559 (71.1) 2276 832
2276 1 444 (28.9) 832 2276
332 2821 (56.4) 2514 332
581 (11.6)
778
448
# OF
SITES SITES
FRAGMENTS
FRAGMENT FND5
7TH111 I (GACNNNGTC)
XHO 2 (PGATCQ5
437 493 ( 9.9) 1 689 2182
778 341 ( 6.6) 437 778
1 359 332 ( 6.6) 2 182 2514
1 689 330 ( 6.6) 1359 1 689
2182 1 03 ( 2.1) 334 437
2514 2 ( 0.0) 332 334
476 $003 (99.9) 476 476
1 373 2160 (43.2) 4216 1 37 3
1 384 1 1 02 (22.0) 2267 3369
1470 847 (16.9) 3369 421 S
1482 768 (15.4) 1 482 225 )
2250 86 < 1.7) 1 384 1473
22 6 7 1 7 < 0.3) 2250 ггс 7
3369 1 2 ( 0.2) 1 470 14E2
4216 1 1 ( 0.2) 1 373 1 324
XMN 1 {GAANNNNTTO
5003 (99.9)
2220 2220
THE FOLLOWING SITES DO NOT APPEAR:
ASU 2 (TTCGAA)
BGl 2 (AGATCT)
BST X1 (CCANNNNNNTGG) ECO RV (GAT АТС)
MST 2 (CCTNAGG)
NRU 1 (TCGCGA)
SMA 1 (CCCGGG)
XBA 1 (TCTAGA)
XMA 3 < CGGCC G)
AVA 1 (CQCGPG) BSSH 2 (GCGCGC) CLA 1 (ATCGAT) KPN 1 (GGTACC) NAE 1 (GCCGGC) PAER7 1 (CTCGAG) SST 1 (GAGCTC) XHO 1 (CTCGAG)
BCL 1 (TGATCA) BST E2 (GGTNACC) ECA 1 (GGTNACC) MLU 1 (ACGCGT ) NAR 1 (GGCGCC)
SAL 1 (GTCGAC) -.SST 2 ( CCGCGG ) XMA 1 (CCCGGG)
Приложение 2 Нуклеотидная последовательность pRSV neo
BST E 2 (GGTNACC) ‘ECA 1 (GGTNACC) PAER7 1 (CTCGAG) SST 2 (CCGCGG) XHO 1 (CTCGAG)
BST X1 (CCANNNNNNTGG) ECO RV (GATATC)
SAL 1 (GTCGAC)
STU 1 (AGGCCT)
CLA KPN 1 SST 1
1 (ATCGAT) (GGTACC) (GAGCTC)
XBA 1 (TCTAGA)
___________10. ____ 20 ______ 30 40 5Q. ________ 60
CTTGGAGGTG CACACCAATG TGGTGAATGG TCAAATGGCG TTTATTGTAT CGAGCTAGGC
7Q. _____ .80 9?_____________ 100 110 120
ACTTAAATAC AATTATCTCT GCAATGCGGA ATTCAGTGGT TCGTCCAATC CATGTCAGAC
130 14 0 1 50 ______ 3 6 0 1 70 180
CTGTCTGTTG CCTTCCTAAT AAGGCACGAT CGTACCACCT TACTTCCACC AATCGGCATG
_ 190 2D0 210 220 230 240
CACGGTGCTT TTTCTCT-CCT TCTAAGGCAT GTTGCTAACT CATCGT7ACC ATGTTGCAAG
29—196
449
250 260 270. _280 29? J03
ACTACAAGTG TATTGCATAA GACTACATTT CCCCCTCCCT ATGCAAAAGC GAAACTACTA
310 ____ 320 ________?30 340 150 360T
TATCCTGAGG GGACTCCTAA CCGCGTACAA CCGAAGCCCC GCTTTTCGCC TAAACACACC
370 ___ 580 _ 390 _________ 400 _________4Ц _ 42tt
•CTAGTCCCCT CAGATACGCG TATATCTGGC CCGTACATCG CGAAGCAGCG CAAAACGCCT
______4?0 ________440 ________450 4-60 470 _________48fr
AACCCTAAGC AGATTCTTCA TGCAATTGTC GGTCAAGCCT IGCCTTGTTG TAGCTTAAAT
____ 490 _ 500 ____510 520 530 54ft
TTTGCTCGCG CACTACTCAG CGACCTCCAA CACACAAGCA GGGAGCAGAT ACTGGCTTAA-
550 ___ 560 ______Slfl _________585. _____ 590 600:
CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA
_______61 0 620 ____?31 ____________640 _6.50 660
CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC-
__________670 6M ________ 690 700 71 0 720
GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAG& CGGTAATAC&
730 _______ 740 750 760 77Д _______ 78JL
GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA.
__________790 8J2JJ ________ 310 820 830 840s
GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA-
_______850 860 __ 370 _____ 880 890 900
CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
_______91 0 920 ________530 _ ____940 950 __9?0
